分別依次使用1M NaOH 溶液和1M NaCl 溶液沖洗20 CV；然后用平衡緩沖液平衡50 CV；按10 CV/min的速度上樣，收集UV-280處的吸收峰，上樣完成后，相同的流速下使用平衡緩沖液繼續沖洗，直到UV-280吸收值到基線，同時收集UV-280處吸收峰。
Flushing 1 Mol NaOH solution and 1 Mol NaCl solution 20CV seperately; With buffer liquid equilibrium 50 CV and on sample in the speed of 10 CV/min,collecting the absorption peak of UV-280,after sampling, using buffer liquid continue on flushing with same speed until the UV-280 absorption value to baseline and collecting the absoption peak of UV-280 as well.
在150L流加工藝建立中，工作體積XXL，接種密度XX/ml，控制Temp XX℃，DO XX%，Agit前期XXrpm，pH前期XX，后期XX，Gas前期XX SLPM。當活細胞密度大于XX/ml，進行第一次流加；當活細胞密度大于XX/ml，進行第二次流加；在第二次流加培養2天后，進行第三次流加，當細胞活性降至XX%，結束培養。
During the Fed-batch process of 150L,the volume of work is XXL,Inoculaton density is XX/ml,controlling the temperaure to XX℃,DO XX %,Agit XXrpm on earlier stage,PH XX on earlier stage and XX on later period,Gas is XX SLPM on earlier stage . When the viable cell density is geater than XX/ml we’ll start the first Fed-batch process, When the viable cell density is geater than XX/ml then begin the second Fed-batch process next after two days of cultivation,we will continue the third Fed-batch process, the whole cultivation will be end up with the cell competency down to XX%.
 
為了確證表達產物一級結構的正確性，企業自行建立了高效液相色譜與質譜聯用肽圖技術：先將XXXX蛋白經過變性、還原和烷基化，再通過胰蛋白酶將其切成不連續的多肽片段，然后利用高效液相色譜將酶切得到的所有肽段進行分離，再連接進入質譜分析每個肽段的準確分子量，通過二級質譜確認各肽段的氨基酸序列。綜合所有鑒定出的肽段序列后與理論蛋白全序列比對，從而計算出蛋白總氨基酸序列覆蓋率。
In order to express the correctness of the product primary structure,enterprise build the HPIC and peptide mapping technology on their own: first denaturate,return and alkylate the xxxx protein,then cut it into disconnected peptide fragment by trypsase,next separate all the atriopetion which cut with HPIC enzyme, final connect all the accurate molecular weight of each atriopetion which join in mass spectrum analysis  and confirm the amino acid sequence of each atriopetion by secondary mass spectrum.  After comparing all the confirmed atriopetion and theory of protein sequence then calculate the coverage rate of otal amino acid sequence of proteins.

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